Pada saat pemanasan media diharapkan tidak menggunakan suhu yang terlalu tinggi. Untuk alasan kepraktisan biasanya pembuatan media langsung dengan sekala besar dalam satu kali sterilisasi agar saat dibutuhkan stok media yang ada tinggal dipanaskan kembali.Weigh and dissolve the components: Measure out the appropriate quantities of Just about every
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The cholinergic neurons inside the PPN and LDT control the two SNc and VTA neurons via activating acetylcholine receptors in these neurons [eight]. Combining optogenetic and brain slice patch-clamp approaches, Xiao et al. [eight] shown that five–ten s of optogenetic stimulation of cholinergic projections within the PPN and LDT evokes inward cur